The travel giant said the closures are part of plans to “streamline” its UK retail network under an efficiency programme, and to address changing customer behaviour.
The closures will result in Thomas Cook’s retail estate reducing to 566 shops.
The company pointed to the fact that more and more holidaymakers are switching online, with web bookings accounting for 64% of all Thomas Cook bookings in the UK last year.
A consultation process with the affected staff and unions has begun.
Thomas Cook’s chief of tour operating, Will Waggott, said: “Today’s announcement reflects the wider challenges seen on the high street, with more and more customers choosing to book online.
“These measures will help us to drive greater efficiencies across Thomas Cook so that we relentlessly focus our resources in those areas that give us the greatest opportunity to make a difference to customers in our core holiday offering.
“Looking ahead, we will be working to ensure that Thomas Cook is fit for the future, putting a rigorous focus on costs in a competitive environment while giving customers more reasons to holiday with the strongest brand in travel.”